Activation of TRPV1 Prevents OxLDL-Induced Lipid Accumulation and TNF-α-Induced Inflammation in Macrophages: Role of Liver X Receptor α
Figure 6
Treatment with TRPV1 agonists increases the activation of LXRα in macrophages. (a) BMDMs were pretreated with capsazepine (10 μM) for 1 h then incubated with evodiamine (500 nM), capsaicin (10 μM), or T0901317 (10 μM) for 6 h. Western blot analysis of nuclear protein level of LXRα and Histone H1 as a normalization control. (b) Macrophages were treated with vehicle, evodiamine (500 nM), capsaicin (10 μM), or T0901317 for 6 h, then immunoprecipitated with anti-LXRα or rabbit IgG. PCR amplification involved specific primers for the ABCA1 gene promoter. The amplified DNA products were separated by electrophoresis with 2% agarose gel. (c) Macrophages were transfected with plasmid phABCA1-Luc or phABCA1-DR4 m-Luc for 24 h, treated with vehicle, evodiamine (500 nM), capsaicin (10 μM), or TO901317 (10 μM) for 24 h, then lysed for Luc activity assays with renilla activity as an internal control. Data are mean ± SD from 5 independent experiments. * versus vehicle-treated cells, # versus phABCA1-Luc-transfected cells with evodiamine or capsaicin treatment.
