Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia

Figure 1

Intrathecal injection of NMDA (2 nmol, ■) causes thermal hyperalgesia when compared to vehicle ◊, and this response is blocked by FeTM-4-PyP⁵⁺ in a dose-dependent manner (0.5 nmol (▲), 1 nmol (●), and 2 nmol (□), given intrathecally 15 min before NMDA). Intrathecal injection of FeTM-4-PyP⁵⁺ alone (◆, 2 nmol) did not exert any effect. Results are expressed as mean ± SEM for 8 rats; ^†𝑃<0.001 compared to vehicle and *𝑃<0.001 compared to NMDA alone.