Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia
Figure 4
NMDA (2 nmol) induced thermal hyperalgesia was also associated with nitration of the glutamate transporter GLT1 ((a)–(c)). At the time at which hyperalgesia was at its peak (40 minutes), immunoprecipitation analysis revealed that FeTM-4-PyP5+ (2 nmol given 15 min before NMDA) reduced the NMDA mediated nitration of GLT1 at the level of the spinal cord ((a), (b)). Immunoprecipitation data are representative of at least 6 gels from 3 different animals performed on different days. Bar graph in (b) represents quantification by densitometric analysis. No difference for GLT-1 or β-actin expression was detected among the lanes in these conditions. † compared to vehicle and * compared to NMDA alone.
