Celecoxib Improves Host Defense through Prostaglandin Inhibition during Histoplasma capsulatum Infection
Figure 5
Celecoxib treatment reduces inflammatory cells in the lung parenchyma. At 2, 14, and 28 days after H. capsulatum infection, with or without celecoxib treatment, the phenotype of the cells present in the lung parenchyma was determined. The cells were marked with antibodies to GR-1 (a), MAC-3 (b), CD11c (c), CD4 (d), or CD8 (e). Myeloid and lymphoid populations were gated using forward/side scatters. A group of noninfected mice receiving water (solid line) and noninfected mice treated with celecoxib (dotted line) were used as controls. The lung tissues were also processed and stained with H & E to identify leukocyte infiltration after 2 (f, g), 14 (h, i), and 28 days (j, k). The lung sections from H. capsulatum-infected mice receiving water p.o. (f, h and j) or celecoxib (g, i and k). Magnification: 100x. The data are presented as the means ± SEM representative of three independent experiments . One-way ANOVA (Tukey's multiple comparison test) was used. *PBS + H2O versus Hc + H2O or versus Hc + celecoxib; #Hc + H2O versus Hc + celecoxib .
