Retinal Pigment Epithelial Cells Express a Functional Receptor for Glucagon-Like Peptide-1 (GLP-1)
Figure 4
GLP-1R silencing abrogates GLP-1-induced signalling pathway activation. ARPE-19 cells were transfected with scrambled (scr) siRNA control or GLP-1R siRNA and treated with 10 nmol/L GLP-1 for 10 minutes. Cells were then lysed and tested with antibody anti-GLP-1R, anti-phPKB, or anti-phERK1/2. Membranes were stripped and reprobed, respectively, with anti-βactin, anti-PKB, or antiERK1/2-antibodies. (a) Representative western blot analyses of three different experiments are shown. (b) Quantification of densitometries of western blot bands of GLP-1R. Data were expressed as mean ± SE of fold induction relative to β-actin (). versus scr siRNA CTR. (c) Quantification of densitometries of western blot bands of phPKB. Data were expressed as mean ± SE of fold induction relative to total PKB (). versus respective CTR. (d) Quantification of densitometries of western blot bands of phERK1. Data were expressed as mean ± SE of fold induction relative to total ERK1 (). versus CTR. (e) Quantification of densitometries of western blot bands of phERK2. Data were expressed as mean ± SE of fold induction relative to total ERK2 (). versus CTR.
