VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue
Figure 2
VEGF neutralization prevents CM- and OA-induced proliferation. Cells were treated with 250, 500, and 1000 pg/mL VEGF (a), CM, OA and CMOA (b and d), and accordingly VEGF, OA, and VEGFOA (c) as described in the legend to Figure 1 in the presence or absence of a specific neutralizing VEGF antibody for 24 h. (a–c) BrdU incorporation into DNA was determined as described in the legend to Figure 1. Data are expressed relative to control. (d) Total cell lysates were resolved by SDS-PAGE and immunoblotted with a specific VEGF-R1 antibody. For VEGF-R1, lanes were excised from a single western blot and displayed in the present order. Data are mean values ± SEM of three independent experiments. All data were normalized to the level of actin expression and are expressed relative to the control. * compared to untreated control or designated data.
