Cockroach Allergen Bla g 7 Promotes TIM4 Expression in Dendritic Cells Leading to Th2 Polarization
Figure 3
rBla g 7 induced release of IL-13 from dendritic cells (DCs). DCs were incubated with various concentrations of rBla g 7 (ng/mL) with or without its specific blocking antibody (Ab-Bg 7, 10 ng/mL), or with LPS for 48 h at 37°C. The levels of IL-12 (a) and IL-13 (c) in the culture supernatant were determined by ELISA, and the expression of IL-12 (b) and IL-13 (d) mRNAs was assessed by real-time PCR. The influence of TIM4, CD80, and CD86 blocking antibodies on rBla g 7 provoked IL-13 release was also examined (e). The data were represented as the mean ± SEM for four separate experiments. in comparison with medium alone control. compared with the response to the corresponding uninhibited control.
