An Asp49 Phospholipase A₂ from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E₂ Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages

Figure 3

p38MAPK, PI3K, and PKC phosphorylation induced by MT-III in isolated peritoneal macrophages. Resident peritoneal macrophages (1×10⁶ cells) were incubated during 1, 5, and 15 min with MT-III (0.4 μM). (a, d, and g) Western blotting of p-p38MAPK, p38MAPK, p-PI3K, PI3K, p-PKC, PKC, and 𝛽-actin (loading control). (b, c, e, f, h, and i) Densitometric analysis of immunoreactive band intensities. Results are expressed as mean ± SEM from 3 experiments. *𝑃<0.05 as compared to time 0.

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