Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents
Figure 4
The antinociceptive effect of bufalin. (a) Bufalin (0.15, 0.3, or 0.6 mg/kg), the reference drug morphine (5 mg/kg), and the vehicle were administered to mice 30 min before a peritoneal injection of acetic acid. The numbers of abdominal writhes were recorded. The inhibition rate of the writhes for 20 min following acetic acid injection was calculated (). (b) ELISA was used to test TNF-α and IL-1β produced by peritoneal cells harvested from peritoneal cavity of animals that underwent acetic acid-induced writhing tests (). Effects of bufalin and morphine on the licking time of the formalin test in mice during the first (0–5 min) (c) and second phases (15–30 min) (d) (). (e) The hot plate latency was measured at 0, 30, 60, and 120 min after administration of bufalin, morphine, or vehicle (). Data are expressed as the mean S.E.M., < 0.05, < 0.01, and < 0.001, compared with the vehicle group.
