MSH Blunts Endotoxin-Induced MuRF1 and Atrogin-1 Upregulation in Skeletal Muscle by Modulating NF-B and Akt/FoxO1 Pathway

Table 1

Primers for real-time PCR.

Gene	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Product bp
18S	GGTGCATGGCCGTTCTTA	TCGTTCGTTATCGGAATTAACC	60
TNF	TGAACTTCGGGGTGATCG	GGGCTTGTCACTCGAGTTTT	122
FoxO1	TCAGGCTAGGAGTTAGTGAGCA	GGGGTGAAGGGCATCTTT	95
Atrogin-1	GAACAGCAAAACCAAAACTCAGTA	GCTCCTTAGTACTCCCTTTGTGAA	74
MuRF1	TGTCTGGAGGTCGTTTCCG	ATGCCGGTCCATGATCACTT	58
IGF-I	GCTATGGCTCCAGCATTCG	TCCGGAAGCAACACTCATCC	62
IGFBP-3	GGAAAGACGACGTGCATTG	GCGTATTTGAGCTCCACGTT	78