Generation of IL-8 and IL-9 Producing CD4+ T Cells Is Affected by Th17 Polarizing Conditions and AHR Ligands
Figure 7
FICZ changes the expression of the Th17 associated microRNA miR-326. (a) The expression of microRNA hsa-miR-326 in CD4+ T cells cultured with anti-CD3/anti-CD28 (○) and with Th17 polarizing conditions (●) for 1, 3, and 5 days was measured by real-time PCR. Data were normalized to the expression of hsa-SNORD-44 and the microRNA expression at day 0 using the Ct-method. Data are shown as mean ± SEM; *represents significance, , unpaired Student’s -test after log-transformation, and = 5–7. (b) The expression of microRNA hsa-miR-326 in CD4+ T cells cultured with anti-CD3/anti-CD28 and Th17 polarizing conditions in the presence (●) of the AHR ligands FICZ (100 nM), TCDD (5 nM), and B[a]P (100 nM) for 1, 3, and 5 days was measured by real-time PCR. CD4+ T cells treated with anti-CD3/anti-CD28, Th17 polarizing conditions, and DMSO (0.05%) were used as control (○). Data were normalized to the expression of hsa-SNORD-44 and the microRNA expression at day 0 using the Ct-method. Data are shown as mean ± SEM; *represents significance, , unpaired Student’s -test after log-transformation, and = 5–7.
