Decreased Expression of the Aryl Hydrocarbon Receptor in Ocular Behcet’s Disease
Figure 1
AhR mRNA expression is decreased in active BD patients and AhR activation by FICZ and ITE inhibits IFN-γ and IL-17 and induces IL-22 production by PBMCs. (a) AhR mRNA was evaluated in PBMCs from active BD patients (), inactive BD patients (), and normal controls () by real-time PCR and normalized to β-actin. The Kruskal-Wallis test and Mann-Whitney test were used to analyze the statistical difference between the various groups. Data are expressed as mean ± s.e.m. (b–g) PBMCs from active BD patients () and normal controls () were stimulated with anti-CD3/CD28 in the presence or absence of FICZ (100 nmol/L) or ITE (100 nmol/L) for 3 days. The supernatants were harvested for detection of IFN-γ (b and e), IL-17 (c and f), and IL-22 (d and g) by ELISA. Paired-sample t test or Wilcoxon’s matched-pairs test for related samples and Independent-Sample t test for independent samples were used for statistical analyses. , .
