IL-1β and TNFα Promote Monocyte Viability through the Induction of GM-CSF Expression by Rheumatoid Arthritis Synovial Fibroblasts

Figure 4

SF conditioned media do not regulate the expression of typical M1 or M2 surface markers. CD14+ monocytes were cultured 4 days in presence of medium with 10% FBS without (not stimulated cells) or with IFNγ (50 ng/mL; M1) or IL-4 (50 ng/mL; M2a) or IL-10 (50 ng/mL; M2c) or RA SF conditioned media. FACS analysis of the cell surface markers for classical (CD14), nonclassical (CD16), M1 (CD64), M2a (CD200R), and M2c (CD163) macrophages was thus performed. Values are expressed as the ratio of the mean fluorescence intensity (MFI) of the marker on the stimulated cells over MFI of unstimulated cells. Bars represent the mean (± SEM) of 3 independent experiments.