The First Trimester Gravid Serum Regulates Procalcitonin Expression in Human Macrophages Skewing Their Phenotype In Vitro
Figure 2
M1 polarized macrophages significantly upregulate the expression of PCT compared to both M0 and M2 macrophages under stimulation with LPS. (a) PMBCs were differentiated in culture for 7 days and polarized O/N with LPS (100 ng/mL) and IFNγ (500 U/mL) or IL-4 (20 ng/mL) and IL-10 (50 ng/mL) for the M1/M2 polarization. mRNA expression levels of CD80 and IL-1β (M1 markers) and CD206 and IL-10 (M2 markers) were measured by qPCR and normalized to those of the human housekeeping gene 18S. (b) RNA obtained from M0, M1, and M2 macrophages were quantified for PCT and 18S expression by qPCR. The relative amount of mRNA for PCT was normalized with reference to 18S value. Results were expressed as AUs, in which 1 AU represents the value obtained with untreated macrophages used as a positive control. Bars represent the mean ± S.E.M. of at least 3 independent experiments. and (Mann-Whitney test).
