Research Article

The First Trimester Gravid Serum Regulates Procalcitonin Expression in Human Macrophages Skewing Their Phenotype In Vitro

Figure 5

Expression of CD206 and PCT in human macrophages conditioned for 24 hours with PG, E2, and hCG or with the three hormones combined together. (a) The mRNA expression level of CD206 was measured by qPCR and normalized to those of the human housekeeping gene 18S. Results were expressed as AUs, in which 1 AU represents the value obtained with macrophages cultured in NHS used as a positive control. (b) PCT fold increase was expressed as ratio between mRNA levels of LPS stimulated and LPS untreated cells. Bars represent the mean ± S.E.M. of at least 3 independent experiments. (Mann-Whitney test).
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