Feed-Forward Inhibition of CD73 and Upregulation of Adenosine Deaminase Contribute to the Loss of Adenosine Neuromodulation in Postinflammatory Ileitis
Figure 4
Influence of tetrodotoxin (TTX, 1 μM), mibefradil (3 μM), and dipyridamole (0.5 μM) on stimulation-evoked release of purine nucleosides (adenosine plus inosine) from the LM-MP of control and TNBS-injected rat ileum. Drugs were in contact with the preparations for at least 15 min before stimulus application (5 Hz frequency, 3000 pulses of 1 ms duration). Samples collected from the incubation media were analysed by HPLC with diode array detection. Illustrated is the purine nucleosides content of bath samples collected immediately before (Pre-EFS) and after (Post-EFS) stimulation of LM-MP expressed in pmol/mg of tissue. Data represent the means ± SEM of an number of individual experiments. (one-way ANOVA followed by Dunnett’s modified t-test) represents significant differences from the evoked amount of purine nucleosides detected in control conditions where no drugs were added to the preparations.
