Macrophage Trafficking as Key Mediator of Adenine-Induced Kidney Injury

Figure 5

Macrophage recruitment in renal tissue is reduced in CCR5 KO animals, leading to less inflammation. (a) Representative image of the macrophage infiltration in the kidney, evaluated by CD11b positive cells. In panel (b) we can observe the quantification of the total data from panel (a) (^∗𝑃<0.01 versus Control and ^∗∗𝑃<0.05 versus CCR5 KO group). The gene expression of CD68 and the ratio regarding the mRNA levels of arginase-1/iNOS can be checked at panels (c) and (d), respectively (^∗∗𝑃<0.05 versus CCR5 KO and ^∗𝑃<0.05 versus WT). Also, the protein levels of the proinflammatory molecules TNF-α, IL-6, and IL-1β were evaluated in the same animals and the results are shown in panels (e), (f), and (g), respectively (^∗𝑃<0.05 versus control and CCR5 KO groups and ^∗∗𝑃<0.05 versus control).

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