Nucleotides Regulate Secretion of the Inflammatory Chemokine CCL2 from Human Macrophages and Monocytes

Figure 4

UTP induces CCL2 gene transcription in THP-1 monocytes. THP-1 cells were plated in RPMI media containing 1% serum and stimulated with media alone, LPS (1 μg/mL), UTP (10 μM), or UDP (10 μM) for 2 hours. RNA was extracted, reverse transcribed to cDNA, and probed with quantitative PCR primers for chemokines (CCL2, CCL20, CCL3, and CXCL8) and reference genes (β-actin and GAPDH). PCR was performed in triplicate using a Rotorgene 2000. Data was analysed using the method using a threshold of 0.003.