Nucleotides Regulate Secretion of the Inflammatory Chemokine CCL2 from Human Macrophages and Monocytes

Figure 6

UTP induces chemokine gene transcription in human monocytes. Human CD14⁺ monocytes were plated in RPMI media containing 1% serum and stimulated with media alone, LPS (1 μg/mL), UTP (10 μM), or UDP (10 μM) for 6 hours. RNA was extracted, reverse transcribed to cDNA, and probed with quantitative PCR primers for chemokines (CCL2, CCL20, CCL3, and CXCL8) and reference gene (β-actin). PCR was performed in triplicate using a Rotorgene 2000. Data was analysed using the method using a threshold of 0.003 and fold upregulation with respect to the media control is plotted.