The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice
Figure 1
Effect of Rg1 on cell viability and apoptosis. (a) CCK8 assay was performed after incubation of RAW 264.7 cells (2 × 104 cells/mL) with Rg1 (1, 10, and 100 μg/mL) for 24, 48, 72, and 96 h in 96-well plates. (b) RAW 264.7 cells (1.0 × 105 cells/mL) were stimulated with or without the presence of Rg1 (1, 10, and 100 μg/mL) for 96 h in a 6 cm culture plate. Cells were collected and stained with Annexin V and PI and then examined by flow cytometry. (c) FACS histogram of the percentage of early and late apoptosis cells. Values are expressed as the mean ± SD of triplicate experiments. All data are representative of three different experiments.
