The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice
Figure 2
Effects of Rg1 on osteoclast differentiation and resorption pit formation in RANKL-stimulated RAW 264.7 cells. (a) RAW 264.7 cells (2 × 104 cells/mL) were stimulated with RANKL (100 ng/mL) for 96 h with or without the presence of Rg1 (1, 10, and 100 μg/mL). Cells were fixed with 3.7% formalin, permeabilized with 0.1% Triton X-100, and stained with TRAP solution. (b) RAW 264.7 cells (5 × 103 cells/mL) were cultured on bone slices with various concentrations of Rg1 in the presence of RANKL (50 ng/mL). After culturing for 10 days, the dentine slice was recovered from the culture and subjected to visualization of resorption pits. (c) The cells were stained for TRAP, and TRAP-positive multinuclear cells containing more than three nuclei were counted as multinucleated osteoclasts (mOCs). (d) The percentages of resorbed area were determined using Image-J software. Values are expressed as the mean ± SD of triplicate experiments. *, **, and ***.
