The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice

Figure 3

Effect of Rg1 on the mRNA expression of osteoclastic marker genes in RANKL-stimulated RAW 264.7 cells. RAW 264.7 cells (2 × 10⁴ cells/mL) were stimulated with RANKL (50 ng/mL) in the presence of Rg1 (1, 10, and 100 μg/mL) for 96 h in 12-well plates. mRNA expressions of osteoclastogenic marker genes were determined by RT-PCR. Values are expressed as the mean ± SD of triplicate experiments. *, **, and ***.

(a)

(b)

(c)

(d)