The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice
Figure 7
Rg1 resulted in reduced histological inflammation, cartilage loss, and OC formation in CIA mice. (a) Histological evaluations of antiarthritis effects of Rg1 were observed in joint slides stained by HE (original magnification, 200x). Histologic scores in the sections shown in (a). (b) Representative histopathologies of proximal interphalangeal joint, and ankle joint stained with Alcian blue in CIA (original magnification, 200x). Histologic analysis of cartilage in CIA mice after Rg1 therapy. Sections were scored in a blinded manner on a four-point scale as described in Materials and Methods section. A significantly less loss of Alcian blue staining was observed in animals treated with Rg1 than compared to controls, indicating inhibition of cartilage destruction. (c) Trap staining in ankle joints and proximal interphalangeal joint isolated from normal, CIA, and Rg1-treated mice. The expression of Trap significantly decreased in Rg1-treated mice compared with CIA mice. (d) Immunohistochemical staining for cathepsin K in ankle joints and proximal interphalangeal joint isolated from normal, CIA, and Rg1-treated mice. The expression of cathepsin K significantly decreased in Rg1-treated mice compared with CIA mice. Data are the mean ± SD, mice. * versus CIA treated with vehicle.
