Anabolic Properties of High Mobility Group Box Protein-1 in Human Periodontal Ligament Cells In Vitro

Figure 6

HMGB1 stimulation influences PDL cell mineralization. HMGB1 exposure of hPDL cells for 21d resulted in enhanced biomineralization (a). In the absence of a respective substrate, no mineralized nodule formation was observed (A), whereas osteogenic medium (OM) itself (B) as well as OM supplemented with HMGB1 (C) led to the formation silver nitrate positive areas (arrows), which were most evident and spread in dexamethasone treated cultures (D). The semiquantitative assessment of mineralized area versus total area revealed confirming results (b). Data represent the mean ± SD for six independent cultures. , experimental group versus untreated control; , dexamethasone treated group versus HMGB1 treated cultures; , HMGB1 treated cultures versus osteogenic medium alone.

(a)

(b)