Research Article

α-Lipoic Acid Inhibits Helicobacter pylori-Induced Oncogene Expression and Hyperproliferation by Suppressing the Activation of NADPH Oxidase in Gastric Epithelial Cells

Figure 7

Effect of DPI on ROS levels and cell proliferation of H. pylori-infected AGS cells. The cells were pretreated with DPI for 2 h and cultured in the presence or absence of H. pylori. (a) ROS levels were determined by DCF fluorescence after 30 min of H. pylori infection. The levels of ROS trapped in the cells treated without DPI and cultured in the absence of H. pylori are considered as 100%. (b) Viable cell numbers were determined by trypan blue exclusion assay for the indicated time period. (c) The cells were treated with thymidine at 24 h after H. pylori infection and incubated for 8 h. The amount of thymidine incorporation of AGS cells cultured in the absence of H. pylori is considered as 100%. versus corresponding none (the cells cultured in the absence of H. pylori); + versus corresponding H. pylori control (the cells cultured in the presence of H. pylori and treated without DPI).
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