A Natural Flavonoid Glucoside, Icariin, Regulates Th17 and Alleviates Rheumatoid Arthritis in a Murine Model

Figure 3

Icariin decreases Th17 cells and represses IL-17 production in vitro. CD4⁺ T cells from the spleens of mice were cultured under Th17 cell-inducing conditions with or without icariin (10 μM/L). (a) After 72 hours of culture, expression of IL-17 mRNA in the cell was determined using PCR. (b) The concentration of IL-17 in culture supernatants of mouse serum was measured using ELISA. (c) The mRNA expression of molecules that are associated with Th17 cell differentiation, including retinoic acid receptor-related orphan nuclear receptor γt (RORγt), aryl hydrocarbon receptor, interferon regulatory factor 4, and CCL20, was determined using real-time PCR. versus all other groups, .

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