A Natural Flavonoid Glucoside, Icariin, Regulates Th17 and Alleviates Rheumatoid Arthritis in a Murine Model

Figure 4

Icariin inhibits osteoclastogenesis in mice. (a) The number of TRAP⁺ cells in synovial tissue sections from the joints of mice with CIA. (b) Osteoclast precursor cells from bone marrow-derived monocytes/macrophage (BMM) cells of normal mice were further cultured in the presence of M-CSF (10 ng/mL) together with RANKL (50 ng/mL) with or without icariin. The number of TRAP⁺ cells and percentage of pit area were determined. (c) The mRNA expression of various osteoclastogenic markers was analyzed using real-time PCR. (d) The protein level of various osteoclastogenic markers was analyzed using Western blot. versus all other groups. .

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