Roles of 5-Lipoxygenase and Cysteinyl-Leukotriene Type 1 Receptors in the Hematological Response to Allergen Challenge and Its Prevention by Diethylcarbamazine in a Murine Model of Asthma

Figure 1

Effect of 5-LO deficiency on the eosinophilia of bone-marrow in vivo in OVA-sensitized and -challenged mice. PAS and ALOX mice were sensitized with OVA and challenged i.n. (a, b) or i.p. (c–f) with saline (SAL) or OVA. Bone-marrow was harvested 48 h after challenge. Data for (a) and (b) (mean + SEM; /7/9/9 in the four groups, from left to right) show (a) numbers of EPO+ cells in freshly collected femoral bone-marrow; (b) numbers of total nucleated cells in the same samples. Data for (c) and (d) (mean + SEM; /8/8/8) show (c) numbers of EPO+ cells in bone-marrow; (d)–(f) numbers of EPO+ cells (d), neutrophils (e), and total leukocytes (f) in peritoneal lavage fluid. All significant differences are indicated.

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