Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
Figure 3
The mRNA and protein level of PRDX1 was determined by western blotting (a), real-time PCR (b), and IHC ((c) and (d)), respectively. (a) Expression of PRDX1 protein in lung tissues of ALI and control rats by western blotting. β-Actin was used as a loading control. Data are reported as relative densitometry of the PRDX1 over β-actin in bar graphs. There is no statistical significance between Control-2 and Control-1, versus Control-1. (b) Real-time PCR analysis of PRDX1 mRNA expression in the ALI and control rats. (c) PRDX1 expression levels were upregulated in ALI rat lung tissue in comparison to the control rat lung tissue as examined by IHC, original magnification, ×200. (d) The average MOD of PRDX1 staining between the ALI and control rat lung tissues was statistically quantified. (b) and (d): rats/group. .
