Interleukin-1 Receptors Are Differentially Expressed in Normal and Psoriatic T Cells
Figure 2
IL-1 receptor mRNA expression is different in psoriatic T cells compared to healthy counterparts. Healthy and psoriatic effector (Teff) and regulatory (Treg) T cells were separated from PBMC fractions. Teff cells were isolated via negative selection method by magnetic beads. Treg cells were sorted from CD4+CD25+ population using anti-CD127 labelling; sorting gate was set for cells (a). Isolated Treg and Teff cells were activated with anti-CD3/CD28 coated beads for indicated times and mRNA expression changes of IL-1 receptor isoforms were determined by real-time RT-PCR at 0 h baseline control and 1 h, 6 h, and 24 h after CD3/CD28 stimulation. Comparison of the expression of IL-1R1 (b), IL-1R2 (c), and sIL-1R2 (d) genes in T cells from healthy (, black circles) and psoriatic samples (, white circles); mean values are indicated by solid and dashed lines, respectively. Gene expression values are represented as arbitrary numbers normalised to the expression of 18S rRNA gene. *—significant difference () between healthy and psoriatic samples and significant difference () between baseline and activated mRNA levels of healthy (#) and psoriatic (&) samples.
