Chemokine CXCL16 Expression Suppresses Migration and Invasiveness and Induces Apoptosis in Breast Cancer Cells

Figure 1

CXCL16 expression in breast cancer cell lines with diverse phenotypic characteristics. (a) CXCL16 mRNA and total protein were highest in MCF-7 and lowest in MDA-MB-231, while noncancerous human mammary epithelial cells MCF-10A faintly expressed CXCL16. ^∗𝑃<0.05 compared with SK-BR-3. ^◆𝑃<0.01 compared with MCF-7. ^#𝑃<0.05 compared with MCF-7. (b) TM-CXCL16 expression in breast cancer cell lines. MCF-7 cells expressed the most TM-CXCL16, while MDA-MB-231 cells barely expressed TM-CXCL16. ^▼𝑃<0.01 compared with SK-BR-3. ^◆𝑃<0.01 compared with MCF-7. (c) and (d) Migratory ability and invasiveness varied by breast cancer cell line. MDA-MB-231 possessed increased migratory ability and invasiveness, while MCF-7 was weakest. ^▼𝑃<0.01 compared with SK-BR-3. ^◆𝑃<0.01 compared with MCF-7. (e) Proliferation of three breast cancer cell lines. MDA-MB-231 proliferated fastest; no difference was observed between SK-BR-3 and MCF-7. ^▼𝑃<0.01 compared with SK-BR-3. ^◆𝑃<0.01 compared with MCF-7.

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