Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells
Figure 4
The effect of NFκB inhibition on airlifted IOBA-NHC TLR expression. (a) Relative activity of p65 in submerged and airlifted IOBA-NHC cells with and without 5-ASA. p65 activity in submerged monolayer cells without 5-ASA was set as 1, and the activities of other cell conditions were compared to it. Asterisks () denote significant difference () between airlifted and submerged cells under the same treatment. (b) Fold changes of selective TLR mRNA expression by qPCR. Individual TLR expression in submerged monolayer cells without 5-ASA was used as control to calculate the fold change. Asterisks () denote significant difference () between airlifted and submerged cells under the same treatment. All experiments were repeated three times, and the data were averaged. error bars represent the standard deviation of the averaged results.
