Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells
Figure 5
TNF-α (10 ng/mL), IL-1α (10 ng/mL), IL-1β (10 ng/mL), IL-6 (10 ng/mL), IL-8 (10 ng/mL), and IFN-γ (40 ng/mL) stimulated TLR1 and TLR2 mRNA expression in submerged primary conjunctival epithelial cells with and without 50 ng/mL LPS. TLR expression in cells cultured in normal medium was used as reference for the calculation of fold increase. Open bars represent the averaged increase when cells were stimulated with cytokine alone. Solid black bars represent the averaged increase when cells were costimulated with indicated cytokines and LPS. The experiments were repeated in three different batches of cells, and the averaged results were presented. error bars represent the standard deviation of the averaged results. Asterisks () denote significant difference when cytokine alone was compared to that without stimulation, and (†) denotes significant difference when cytokine alone was compared to that with LPS.
