Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells

Figure 7

Wounding stimulated TLR expression in submerged primary conjunctival epithelial cells. Confluent cells were scratched by sterile surgical blade at about 1 cm interval and harvested 24 hrs later for RNA extraction and qPCR analysis. The experiment was repeated in 3 different batches of primary cells, and the averaged changes were shown. 𝑌 error bars represent the standard deviation of the averaged results. The increase was statistically significant for each TLR.