Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells

Table 1

ΔCt of individual TLR mRNA in submerged and airlifted primary human conjunctival epithelial cells, immortalized human conjunctival epithelial cells (IOBA-NHC), and immortalized human corneal epithelial cells (HCET).

mRNA	Primary conjunctival epithelial cells (𝑛=3)		IOBA-NHC cells		HCET cells
	Submerged	Airlifted	Submerged	Airlifted	Submerged	Airlifted
TLR1	10.97 ± 1.08	6.27 ± 0.72	7.02 ± 0.36	4.77 ± 0.42	10.58 ± 0.03	8.55 ± 0.14
TLR2	10.51 ± 1.84	6.14 ± 1.32	8.92 ± 0.24	5.17 ± 0.56	5.93 ± 0.38	1.98 ± 0.18
TLR3	9.13 ± 1.93	4.88 ± 1.23	4.69 ± 0.18	2.57 ± 0.38	4.63 ± 0.17	2.65 ± 0.26
TLR4	14.93 (𝑛=1)	9.74 ± 1.01	3.06 ± 0.14	1.57 ± 0.30	7.25 ± 0.04	4.71 ± 0.10
TLR5	10.61 ± 2.08	6.28 ± 1.13	10.85 ± 1.79	6.75 ± 0.18	5.64 ± 0.17	4.36 ± 0.09
TLR6	12.41 ± 1.02	8.51 ± 0.53	5.39 ± 0.48	4.48 ± 0.51	ND	ND
TLR9	13.32 ± 1.19	10.36 ± 0.78	9.54 ± 0.73	6.35 ± 0.16	10.10 ± 0.46	8.21 ± 0.79

ΔCt was calculated using 𝛽-actin as internal control. Data was presented as average ± standard deviation. The experiment was performed three times for each condition. For primary cells, the results shown were the averaged 𝛿Ct of three different batches of cells. “Submerged” refers to submerged-cultured cells at 80–90% confluence. “Airlifted” refers to airlifted culture at 10 days. ND stands for nondetected.