High Expression of Ecto-Nucleotidases CD39 and CD73 in Human Endometrial Tumors
Figure 2
(a) Enzyme in situ histochemistry in endometrioid ((A), (B)) and in serous ((C), (D)) endometrial adenocarcinomas in the presence of ADP as substrate, alone ((A), (C)) or together with the inhibitor NF279 ((B), (D)). Strong ADPase activity was detected in the stroma of both types of tumors ((A), (C) dark brown deposits), while the activity was drastically reduced in the presence of the inhibitor ((B), (D)). Scale bars = 100 μm. (b) ADPase enzyme activity in tissue homogenates of endometrioid () and serous () adenocarcinomas. Experiments were performed in triplicate for each sample. Data are represented in arbitrary units (AU). *Significant differences at . (c) ADPase in situ activity in endometrial touch preparations from human endometrioid adenocarcinomas using ADP as substrate (A) and in the absence of substrate (B). Scale bars = 50 μm.
