High Expression of Ecto-Nucleotidases CD39 and CD73 in Human Endometrial Tumors
Figure 3
(a) Immunolocalization of ecto-5′-nucleotidase/CD73 ((A), (D)) and AMPase in situ histochemistry ((B), (C), (E), and (F)) in endometrioid ((A)–(C)) and serous ((D)–(F)) endometrial adenocarcinomas. CD73 was immunodetected in glandular epithelial cells and in the stroma of both types of adenocarcinomas ((A), (D)). The AMPase activity mirrors the immunolocalization ((B), (E)). The activity was abolished in the presence of α, β-meADP ((C), (F)). Insets correspond to control experiments performed in the absence of nucleotide (no AMP). Scale bars = 50 μm ((A)–(C), (F), insets) and 250 μm ((D), (E)). (b) AMPase in situ activity by enzyme histochemistry in endometrial touch preparations. Dark precipitates in (A) correspond to the AMPase activity in the presence of AMP as substrate. The inhibitor α, β-meADP completely abolished this activity (B). Control experiments were performed in the absence of substrate (C). Scale bars = 50 μm.
