The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model
Figure 3
Intratumoral expression levels of cytokines mRNA. An experimental tumor model was generated and treated with the IL-12 gene expression plasmid (asterisks), pcDNA3 plasmid (triangle), PBS (square), or no treatment as control (diamond). Every three days following treatment the mice were sacrificed, the total RNA was extracted, and the cytokines expression profile was determined with by real time RT-PCR. Th2-Th3-type cytokine profiles and no expression of Th1-tye cytokines were detected in the tumor-bearing mice prior to the treatment. A significantly lower expression of Th2-/Th3 cytokines and higher expression of Th1 cytokines were detected after the treatment with IL-12. Results represent the means ± S.E. values of cytokines expression in each 5-mouse group, .
