Preparation and Identification of Per a 5 as a Novel American Cockroach Allergen
Figure 6
(a) Analysis of specific IgE reactivity of recombinant bPer a 5 and iPer a 5 by direct ELISA. The sera were collected from the patients with American CR allergy and healthy control subjects. The values shown are mean ± SEM for the triplicate experiments. * in comparison with those of healthy control subjects. (b) Inhibition of the IgE reactivity to the cockroach extract by bPer a 5 and iPer a 5. CR = American cockroach extract. The values shown are mean ± SEM for the triplicate experiments. * in comparison with the uninhibited control. (c) Western blot analysis of IgE reactivity to bPer a 5 and iPer a 5 in the sera from the patients with American CR allergy. Lanes 1–3: iPer a 5 reacted with the serum from patients 5, 9, and 10. Lanes 4–6: bPer a 5 reacted with the serum from patients 5, 9, and 10. Lanes 7-8: iPer a 5 reacted with the serum from controls 1 and 2. Lanes 9-10: bPer a 5 reacted with the serum from controls 1 and 2.
