GSK-3β Inhibition Attenuates CLP-Induced Liver Injury by Reducing Inflammation and Hepatic Cell Apoptosis

Figure 1

GSK-3β inhibition improves survival and protects against CLP-induced liver injury. The septic mice were treated with SB216763 (25 mg/kg, i.p.) or vehicle (DMSO) at 1 h, 6 h, and 12 h after CLP. (a) Liver samples were harvested at 6 h after CLP and were subjected to western blotting analysis of phosphorylated (serine 9) GSK-3β and phosphorylated glycogen synthases (GS). (b) ALT and AST levels were analyzed as a measure of hepatocellular injury. Data are shown as mean ± SD. , ^# compared with vehicle-treated group. (c) The liver specimens were sampled at 20 h after CLP and stained with hematoxylin and eosin staining (original magnification ×400). Representative images from 6 mice/group were selected. (d) The Kaplan-Meier method was used to determine the difference of survival rate after CLP. value was analyzed by log rank test. compared with vehicle-treated group.

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