TLR2 Elicits IL-17-Mediated RANKL Expression, IL-17, and OPG Production in Neutrophils from Arthritic Mice

Figure 5

RANKL expression and OPG secretion by neutrophils were regulated by TLR2 ligand and IL-17. Purified neutrophils were stimulated as in Figure 3. (a) Representative histograms show RANKL expression on blood neutrophils from nonarthritic and arthritic mice. MFI: mean fluorescence intensity. (b) Graphs indicate that IL-17 increases the frequencies of RANKL⁺ neutrophils in the control group. More RANKL⁺ cells are found after stimulation with TLR2 and IL-17 in arthritic group. (c) IL-17 and zymosan induce OPG secretion by control neutrophils and inhibit OPG production by cells from arthritic mice. Values in (b) and (c) are the mean ± SEM (𝑛=5 animals/group; 3 experiments). *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001, Student’s 𝑡-test.

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