TLR2 Elicits IL-17-Mediated RANKL Expression, IL-17, and OPG Production in Neutrophils from Arthritic Mice

Figure 6

RANKL expression, IL-17, and IFN-γ production of Ly6G⁺ cells in BM or SF. (a) Dot-plot histograms show increased frequencies of Ly6G⁺CD11b⁺ cells in BM of mice with arthritis than in control group. The density plots indicate a higher distribution of RANKL⁺ cells in Ly6G⁺CD11b⁺ population from arthritic group (SSC: side scatter). (b) RANKL-bearing Ly6G⁺ cells accumulated in SF of mice with arthritis. Bars represent the mean ± SEM (𝑛=5 animals/group; 3 experiments). ***𝑃<0.001, Student’s 𝑡-test. (c) Synovial cells from 5 animals per group were pooled and intracellular cytokine production was performed on gated Ly6G⁺ cells. Representative dot-plot histograms show IFN-γ and IL-17 production in Ly6G⁺ cells from SF of mice with arthritis (day 7) (𝑛=5 animals/group; representative from 3 experiments).

(a)

(b)

(c)