The Macrophage Inflammatory Proteins MIP1 (CCL3) and MIP2 (CXCL2) in Implant-Associated Osteomyelitis: Linking Inflammation to Bone Degradation
Figure 4
Generation of osteoclasts by CCL3. (a) Monocytes were cultivated with CCL3 (10 ng/mL); at day 18, giant cells with multiple nuclei (blue) and actin in a ring-like structure were seen (A). These cells also expressed cathepsin K (B red) and TRAP (C). (b) Stimulation with CCL3 induced the induction of NFATc1. A Western blot of cell lysates derived from untreated cells (-) or from cells incubated with RANKL or CCL3 is shown. The loading of the gel was controlled and normalised using actin; the numbers indicate the increase over untreated cells (for technical reasons, sample CCL3 50 ng had run in a distant lane and was cut and added).
