Circulating Th17, Th22, and Th1 Cells Are Elevated in the Guillain-Barré Syndrome and Downregulated by IVIg Treatments

Figure 1

Quantification of circulating CD4⁺IFN-𝛾⁺, Th17 (CD4⁺IL-17A⁺), and CD4⁺IL-22⁺ cells in each group. (a) Representative two-color dot plot analyses of CD4⁺IFN-𝛾⁺, CD4⁺IL-17A⁺, and CD4⁺IL-22⁺ cells after stimulation with PMA and ionomycin in the presence of Brefeldin A from a GBS patient. (b) Frequency of CD4⁺IFN-𝛾⁺, CD4⁺IL-17A⁺, and CD4⁺IL-22⁺ cells on the gated lymphocytes in the FSC/SSC plot in each group: GBS (𝑛=29), RR-MS/R (𝑛=15), VEM (𝑛=17), and HC (𝑛=20). Results are represented as the mean ± SD. Statistical significance was indicated: ^∗𝑃<0.01 and ^∗∗𝑃<0.001.

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