Regulation of Hemichannels and Gap Junction Channels by Cytokines in Antigen-Presenting Cells

<table>Dendritic and B cells of mouse spleen present pannexin1. Immunofluorescence of adult mice spleen cryosections (8 <i>μ</i>m thick) fixed in ethanol (70% v/v) at −20°C for 20 min, mounted in Fluoromount-G and observed in a confocal microscope (Olympus, FluoView FV1000). Pannexin1 (Panx1 in green: primary antibody: rabbit anti-Panx1 antibody and secondary antibody goat anti-rabbit IgG conjugated to FITC) immunoreactivity is shown. Cells were identified by their reactivity to specific antigens as follows: dendritic cells (DCs) by CD11c (blue, monoclonal mouse antibody conjugated to allophycocyanin) and B cells by B220 (red, monoclonal mouse antibody conjugated to phycoerythrin) in a follicle. Arrows denote follicular DCs (arrows). Merge is also shown. Scale bar: 100 <i>μ</i>m.</table>

Mediators of Inflammation

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Figure 3

Figure 3: Regulation of Hemichannels and Gap Junction Channels by Cytokines in Antigen-Presenting Cells 