Regulation of Hemichannels and Gap Junction Channels by Cytokines in Antigen-Presenting Cells

Figure 5

Expression of pannexin1 (Panx1) in Kupffer cells. Immunofluorescence analysis of liver cryosections (8 μm thick) obtained from adult wild-type (WT) and Panx1^−/− adult (C57/BL6) mice was performed to analyze reactivity of Panx1 (green, primary antibody: rabbit anti-Panx1 antibody and secondary antibody goat anti-rabbit IgG conjugated to FITC) in ED2 (red: goat polyclonal antibody and secondary antibody mouse anti-goat conjugated to Cy3) positive cells, which correspond to Kupffer cells. Top panels correspond to a liver section of a WT mouse and bottom panels correspond to a liver section of a Panx1^−/− mouse. No specific Panx1 reactivity was detected in Panx1^−/− liver, but ED2 positive cells were evident. DAPI stain was used to visualize nuclei (blue), and merge is also shown. Panx1^−/− mice were kindly donated by Dr. Hanna Monyer (University of Heidelberg, Germany). Bar: 10 μm.