Hyperthermia Differently Affects Connexin43 Expression and Gap Junction Permeability in Skeletal Myoblasts and HeLa Cells
Figure 2
Effect of hyperthermia on Cx43 GJIC in SkMs. (a) LY diffusion between SkMs was measured by the scrape-loaded dye transfer technique (dashed line indicates the scrape margin; arrow shows the measurement direction of the LY diffusion). (b) Kinetics of scrape-loaded LY diffusion under control conditions, measured 3 min after LY application. Raw data were fitted with an exponential decay function. (c) Comparison of scrape-loaded LY diffusion under control and hyperthermia conditions in the presence and absence of XG-102 (4 μM). FD50 was , , , and μm, respectively. in each set of experiments; , ANOVA followed by post hoc Dunnet’s test. Scale bar 100 μm.
