Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
Figure 3
LPS upregulated RAGE expression and proinflammatory cytokines release in OLC-1 culture. (a) Cells were stimulated with LPS as indicated for 6 h. The expression of RAGE was examined by quantitative real-time PCR. (b) Cells were stimulated with LPS for 24 h, and RAGE protein was examined by western blot analysis. A representative blot using antibodies against RAGE and β-actin is shown. RAGE expression was quantified by densitometry and normalized to β-actin. (c) Increased RAGE surface expression by OLC-1 cells following LPS stimulation for 12 h was analyzed by flow cytometry. A representative histogram is shown. (d) Active release of HMGB1 in a time-dependent manner upon stimulation with 100 ng/mL LPS. (e) HMGB1 release in a dose-dependent manner after LPS stimulation for 24 h. IL-1β (f) and IL-8 (g) release in a time-dependent fashion following LPS stimulation. Results are expressed as the means SD from triplicate experiments. , .
