Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
Figure 4
RAGE mediates the effects of LPS-induced HMGB1 release in OLC-1. Cells were transfected with RAGE siRNA suspended in lipofectamine reagent for 48 h. (a) By RT-PCR, RAGE siRNA silences RAGE mRNA expression without affecting house-keeping gene (GAPDH) mRNA expression. A representative experiment is shown, and the RAGE-specific RNA bands are indicated. (b) Cells were transfected with either RAGE siRNA or negative control siRNA and stimulated with LPS for 24 h, and the HMGB1 release in the supernatant was measured by ELISA. Results are expressed as the means SD from duplicate experiments. .
