Ouabain Affects the Expression of Activation Markers, Cytokine Production, and Endocytosis of Human Monocytes
Figure 2
Ouabain dose response curve on the expression of activation and costimulatory molecules by monocytes. Cells were cultured in the absence or presence of 10−7 M, 10−8 M, and 10−9 M of ouabain for 24 hours. After 24 hours, these cells were incubated for 30 minutes with specific antibodies, as previously described. (a) Histograms show the patterns of CD69, CD80, HLA-DR, and CD86 expression on monocytes untreated or treated with different concentrations of ouabain. Values of CD69+ and CD80+ cells and mean fluorescence intensity of HLA-DR+ and CD86+ cells were also added to the histograms. Control and OUA 10−7 M, ; OUA 10−8 and 10−9 M, , with the exception of CD69 experiments (). (b) Data are expressed as the percentage of CD69+ monocytes and lines refer to the median of ten (Control and OUA 10−7 M) or six (OUA 10−8 and 10−9 M) independent experiments. using both repeated measures ANOVA with Dunnett’s posttest (when analyzing only corresponding data) and two-tailed paired -test (when analyzing all data).
